High throughput cell mechanotyping of cell response to cytoskeletal modulations using a microfluidic cell deformation system

Smith, I. M.; Ursitti, J. A.; Majeti, P.; Givpoor, N.; Stemberger, M. B.; Hengen, A.; Banerjee, S.; Stains, J. P.; Martin, S. S.; Ward, C. P.; Stroka, K. M.

doi:10.1101/2024.06.17.599307

bioRxiv · 10.1101/2024.06.17.599307

High throughput cell mechanotyping of cell response to cytoskeletal modulations using a microfluidic cell deformation system

Smith, I. M.·Ursitti, J. A.·Majeti, P.·Givpoor, N.·Stemberger, M. B.·Hengen, A.·Banerjee, S.·Stains, J. P.·Martin, S. S.·Ward, C. P.·Stroka, K. M.

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Abstract

Cellular mechanical properties influence cellular functions across pathological and physiological systems. The observation of these mechanical properties is limited in part by methods with a low throughput of acquisition or with low accessibility. To overcome these limitations, we have designed, developed, validated, and optimized a microfluidic cellular deformation system (MCDS) capable of mechanotyping suspended cells on a population level at a high throughput rate of [~]300 cells pers second. The MCDS provides researchers with a viable method for efficiently quantifying cellular mechanical properties towards defining prognostic implications of mechanical changes in pathology or screening drugs to modulate cytoskeletal integrity.

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Smith, I. M., Ursitti, J. A., Majeti, P., Givpoor, N., Stemberger, M. B., Hengen, A., Banerjee, S., Stains, J. P., Martin, S. S., Ward, C. P., Stroka, K. M.. 2024-06-19. High throughput cell mechanotyping of cell response to cytoskeletal modulations using a microfluidic cell deformation system. https://doi.org/10.1101/2024.06.17.599307

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