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bioRxiv · 10.1101/2024.06.16.599202

The comparison of single-cell RNA sequencing platforms based droplets

Zhong, Y.·Wang, L.
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Abstract

Single-cell sequencing enables to reveal cellular heterogeneity and discover new cellular subpopulations. In terms of the strategy of single-cell sequencing, the main methods are based with combinatorial index, microwell and microfluidic. Due to the simplicity, methods based droplets are widely used for single-cell sequencing for multi-omics. Therefore, in order to facilitate researchers to choose a suitable platform to meet their application scenarios, we compared several commercial platforms: the Chromium X platform of 10x Genomics, the MobiNova-100 platform of MobiDrop, the SeekOne platform of SeekGene, and the C4 platform of BGI. Based the comprehensive assessment of the data analysis, the Chromium X platform shows a excellent performance, closely followed by MobiNova-100 platform. One-Sentence SummaryAs droplet-based single-cell sequencing platforms, Chromium X and MobiNova-100 have comparable data performance.

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Zhong, Y., Wang, L.. 2024-06-17. The comparison of single-cell RNA sequencing platforms based droplets. https://doi.org/10.1101/2024.06.16.599202

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