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bioRxiv · 10.1101/2024.06.09.598118

Interplay of kinetochores and catalysts drives rapid assembly of the mitotic checkpoint complex

Sethi, S.·Piano, V.·Ghetti, S.·Cmentowski, V.·Stege, P.·Musacchio, A.
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Abstract

The spindle assembly checkpoint (SAC) makes mitotic exit contingent on completion of sister chromatid biorientation, but how this coordination is achieved in practice remains poorly understood. Kinetochores, megadalton chromosome attachment sites to spindle microtubules, contribute to SAC signaling. However, it is unclear whether kinetochores are mere docking sites for SAC proteins, or further contribute to co-orientation of SAC catalysts, including MAD1:MAD2 and BUB1:BUB3, to facilitate SAC signaling. Here, we combined biochemical reconstitutions of kinetochores and the SAC to address this question in vitro. We engineered recombinant kinetochore particles that recruit most SAC components and activate SAC signaling after induction with Rapamycin, and challenged them with a battery of impairing mutants. At approximately physiological concentrations of SAC catalysts, kinetochores were crucially required for rapid assembly of the mitotic checkpoint complex (MCC), the SAC effector. Our observations depict kinetochores as a cradle that catalyzes rapid MCC assembly by concentrating and co-orienting distinct SAC catalysts.

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Sethi, S., Piano, V., Ghetti, S., Cmentowski, V., Stege, P., Musacchio, A.. 2024-06-09. Interplay of kinetochores and catalysts drives rapid assembly of the mitotic checkpoint complex. https://doi.org/10.1101/2024.06.09.598118

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