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bioRxiv · 10.1101/2024.06.08.598075

Predicting RNA Structure and Dynamics with Deep Learning and Solution Scattering

Abstract

Advanced deep learning and statistical methods can predict structural models for RNA molecules. However, RNAs are flexible, and it remains difficult to describe their macromolecular conformations in solutions where varying conditions can induce conformational changes. Small-angle X-ray scattering (SAXS) in solution is an efficient technique to validate structural predictions by comparing the experimental SAXS profile with those calculated from predicted structures. There are two main challenges in comparing SAXS profiles to RNA structures: the absence of cations essential for stability and charge neutralization in predicted structures and the inadequacy of a single structure to represent RNAs conformational plasticity. We introduce Solution Conformation Predictor for RNA (SCOPER) to address these challenges. This pipeline integrates kinematics-based conformational sampling with the innovative deep-learning model, IonNet, designed for predicting Mg2+ ion binding sites. Validated through benchmarking against fourteen experimental datasets, SCOPER significantly improved the quality of SAXS profile fits by including Mg2+ ions and sampling of conformational plasticity. We observe that an increased content of monovalent and bivalent ions leads to decreased RNA plasticity. Therefore, carefully adjusting the plasticity and ion density is crucial to avoid overfitting experimental SAXS data. SCOPER is an efficient tool for accurately validating the solution state of RNAs given an initial, sufficiently accurate structure and provides the corrected atomistic model, including ions. The method is available from: https://github.com/dina-lab3d/IonNet Our pipeline is available for use as a web server: https://bilbomd.bl1231.als.lbl.gov/ Statement of SignificanceUnderstanding the behavior of RNA in solution is critical for deciphering its biological functions, yet predicting its macromolecular conformation remains challenging. While advanced computational methods can predict RNA structures, their accuracy in solution is often limited by the absence of stabilizing ions and the failure to account for RNAs conformational flexibility. This study presents SCOPER, an innovative tool that addresses these challenges by integrating deep-learning-based ion binding site prediction with conformational sampling, offering a more reliable approach to validate and refine RNA structures against experimental SAXS data. We provide our source code and a web server that runs the pipeline.

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BibTeXRIS

Patt, E., Schneidman-Duhovny, D., Hammel, M., Classen, S.. 2024-06-10. Predicting RNA Structure and Dynamics with Deep Learning and Solution Scattering. https://doi.org/10.1101/2024.06.08.598075

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