Global genomics of Aedes aegypti unveils widespread and novel persistent infectious viruses capable of triggering a small RNA response

Gupta, S.; Sharma, R.; Williams, A. E.; Sanchez-Vargas, I.; Rose, N. H.; Zhang, C.; Crosbie-Villaseca, A.; Zhu, Z.; Dayama, G.; Gloria-Soria, A.; Brackney, D. E.; Manning, J.; Wheeler, S. S.; Caranci, A.; Reyes, T.; Sylla, M.; Badolo, A.; Akorli, J.; Aribodor, O. B.; Ayala, D.; Liu, W.-L.; Chen, C.-H.; Vasquez, C.; Acosta, C. G.; Ponlawat, A.; Magalhaes, T.; Carter, B.; Wesson, D.; Surin, D.; Younger, M. A.; Costa-da-Silva, A. L.; DeGennaro, M.; Bergman, A.; Lambrechts, L.; McBride, C. S.; Olson, K. E.; Calvo, E.; Lau, N. C.

doi:10.1101/2024.06.06.597482

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bioRxiv · 10.1101/2024.06.06.597482

Global genomics of Aedes aegypti unveils widespread and novel persistent infectious viruses capable of triggering a small RNA response

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Abstract

The mosquito Aedes aegypti is a prominent vector for arboviruses, but the breadth of mosquito viruses that infects this specie is not fully understood. In the broadest global survey to date of over 200 Ae. aegypti small RNA samples, we detected viral small interfering RNAs (siRNAs) and Piwi interacting RNAs (piRNAs) arising from mosquito viruses. We confirmed that most academic laboratory colonies of Ae. aegypti lack persisting viruses, yet two commercial strains were infected by a novel tombus-like virus. Ae. aegypti from North to South American locations were also teeming with multiple insect viruses, with Anphevirus and a bunyavirus displaying geographical boundaries from the viral small RNA patterns. Asian Ae. aegypti small RNA patterns indicate infections by similar mosquito viruses from the Americas and reveal the first wild example of dengue virus infection generating viral small RNAs. African Ae. aegypti also contained various viral small RNAs including novel viruses only found in these African substrains. Intriguingly, viral long RNA patterns can differ from small RNA patterns, indicative of viral transcripts evading the mosquitoes RNA interference (RNAi) machinery. To determine whether the viruses we discovered via small RNA sequencing were replicating and transmissible, we infected C6/36 and Aag2 cells with Ae. aegypti homogenates. Through blind passaging, we generated cell lines stably infected by these mosquito viruses which then generated abundant viral siRNAs and piRNAs that resemble the native mosquito viral small RNA patterns. This mosquito small RNA genomics approach augments surveillance approaches for emerging infectious diseases.

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Gupta, S., Sharma, R., Williams, A. E., Sanchez-Vargas, I., Rose, N. H., Zhang, C., Crosbie-Villaseca, A., Zhu, Z., Dayama, G., Gloria-Soria, A., Brackney, D. E., Manning, J., Wheeler, S. S., Caranci, A., Reyes, T., Sylla, M., Badolo, A., Akorli, J., Aribodor, O. B., Ayala, D., Liu, W.-L., Chen, C.-H., Vasquez, C., Acosta, C. G., Ponlawat, A., Magalhaes, T., Carter, B., Wesson, D., Surin, D., Younger, M. A., Costa-da-Silva, A. L., DeGennaro, M., Bergman, A., Lambrechts, L., McBride, C. S., Olson, K. E., Calvo, E., Lau, N. C.. 2024-06-06. Global genomics of Aedes aegypti unveils widespread and novel persistent infectious viruses capable of triggering a small RNA response. https://doi.org/10.1101/2024.06.06.597482

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