Transcription templated assembly of the nucleolus in the C. elegans embryo

Abstract

The nucleolus is a multicomponent structure made of RNA and proteins that serves as the site of ribosome biogenesis within the nucleus. It has been extensively studied as a prototype of a biomolecular condensate whose assembly is driven by phase separation. While the steady-state size of the nucleolus is quantitatively accounted for by the thermodynamics of phase separation, we show that experimental measurements of the assembly dynamics are inconsistent with a simple model of a phase-separating system relaxing to its equilibrium state. Instead, we show that the dynamics are well described by a model in which the transcription of ribosomal RNA actively drives nucleolar assembly. We find that our model of active transcription-templated assembly quantitatively accounts for the rapid kinetics observed in early embryos at different developmental stages, and for different RNAi perturbations of embryo size. Our model predicts a scaling of the time to assembly with the volume of the nucleus to the one-third power, which is confirmed by experimental data. Our study highlights the role of active processes such as transcription in controlling the placement and timing of assembly of membraneless organelles. Significance statementHow membraneless organelles like nucleolus assemble within cells is not well understood. Recent experiments suggest that transcription of ribosomal RNA actively drives nucleolar assembly. Our proposed model of active transcription-templated assembly quantitatively accounts for the rapid kinetics observed in early worm embryos at different developmental stages. Further, it predicts a scaling of the time to assembly with the volume of the nucleus that is confirmed by experimental data. This work describes how active processes such as transcription can control the placement and timing of assembly of membraneless organelles.