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bioRxiv · 10.1101/2024.05.20.594984

Dynamic basis of lipopolysaccharide export by LptB2FGC

Dajka, M.·Rath, T.·Morgner, N.·Joseph, B.
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Abstract

Lipopolysaccharides (LPS) confer resistance against harsh conditions, including antibiotics, in Gram-negative bacteria. The lipopolysaccharide transport (Lpt) complex, consisting of seven proteins (A-G), exports LPS across the cellular envelope. LptB2FG forms an ATP-binding cassette transporter that transfers LPS to LptC. How LptB2FG couples ATP binding and hydrolysis with LPS transport to LptC remains unclear. We observed the conformational heterogeneity of LptB2FG and LptB2FGC in micelles and/or proteoliposomes using pulsed dipolar electron spin resonance spectroscopy. Additionally, we monitored LPS binding and release using laser-induced liquid bead ion desorption mass spectrometry. The {beta}-jellyroll domain of LptF stably interacts with the LptG and LptC {beta}-jellyrolls in both the apo and vanadate-trapped states. ATP binding at the cytoplasmic side is allosterically coupled to the selective opening of the periplasmic LptF {beta}-jellyroll domain. In LptB2FG, ATP binding closes the nucleotide binding domains, causing a collapse of the first lateral gate as observed in structures. However, the second lateral gate, which forms the putative en try site for LPS, exhibits a heterogeneous conformation. LptC binding limits the flexibility of this gate to two conformations, likely representing the helix of LptC as either released from or inserted into the transmembrane domains. Our results reveal the regulation of the LPS entry gate through the dynamic behavior of the LptC transmembrane helix, while its {beta}-jellyroll domain is anchored in the periplasm. This, combined with long-range ATP-dependent allosteric gating of the LptF {beta}-jellyroll domain, may ensure efficient and unidirectional transport of LPS across the periplasm.

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Dajka, M., Rath, T., Morgner, N., Joseph, B.. 2024-05-21. Dynamic basis of lipopolysaccharide export by LptB2FGC. https://doi.org/10.1101/2024.05.20.594984

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