rpfF is not required for X. translucens pv. undulosa pathogenesis

Heiden, N.; Butchacas, J.; Klass, T.; Cohen, S. P.; Roman-Reyna, V.; Merfa, M. V.; Valero David, G. E.; Vargas-Garcia, C.; Olmos, C.; Velez-Negron, Y.; Lewandowski, M. M.; Jacobs, J. M.

doi:10.1101/2024.05.20.594922

bioRxiv · 10.1101/2024.05.20.594922

rpfF is not required for X. translucens pv. undulosa pathogenesis

Heiden, N.·Butchacas, J.·Klass, T.·Cohen, S. P.·Roman-Reyna, V.·Merfa, M. V.·Valero David, G. E.·Vargas-Garcia, C.·Olmos, C.·Velez-Negron, Y.·Lewandowski, M. M.·Jacobs, J. M.

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Abstract

Bacterial cells self-coordinate via a mechanism called quorum sensing. In Xanthomonas species the gene rpfF encodes the quorum sensing autoinducer synthase. Xanthomonas species are divided into two main phylogenetic groups called Clade I and Clade II. The rpf quorum sensing system has been well studied in multiple Clade II Xanthomonas species and deletion of rpfF resulted in a major loss of virulence on susceptible hosts. However, the only Clade I Xanthomonas species in which the rpfF system was previously studied was in the sugarcane pathogen X. albilineans. In X. albilineans the rpf cluster plays a relatively small role in pathogenesis. Xanthomonas translucens pv. undulosa (Xtu) is a Clade I Xanthomonas species that causes bacterial leaf streak (BLS) and black chaff of wheat and barley and has increased as a concern in recent decades. Neither major resistance nor chemical treatments are available to prevent disease caused by Xtu. Interference with rpf bacterial quorum sensing systems has demonstrated some success in other systems. It was unknown whether BLS caused by Xtu could be prevented via quorum sensing interference. We found that Xtu encodes an rpfF homolog and we created an rpfF knockout mutant to study the role of the rpf system in Xtu. We found that the rpfF mutant was unaffected in its pathogenesis as it caused BLS symptoms and multiplied within wheat plants to the same levels as the wildtype strain. The Xtu rpfF mutant grew normally in lag and log phases in vitro, however it exhibited a shorter stationary phase and an early death phase in plant-derived media. The importance of RpfF in Xtus life cycle is unknown, though it appears to carry out a role in population stability. Our research determined that rpfF is not a major Xtu pathogenicity factor. Therefore, we do not recommend the targeting of the rpf quorum sensing system as a preventative treatment for BLS of wheat.

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Heiden, N., Butchacas, J., Klass, T., Cohen, S. P., Roman-Reyna, V., Merfa, M. V., Valero David, G. E., Vargas-Garcia, C., Olmos, C., Velez-Negron, Y., Lewandowski, M. M., Jacobs, J. M.. 2024-05-20. rpfF is not required for X. translucens pv. undulosa pathogenesis. https://doi.org/10.1101/2024.05.20.594922

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