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bioRxiv · 10.1101/2024.05.14.594256

Impact of long-term rapamycin treatment on age-related osteoarthritis in common marmoset

Abstract

PurposeGenetic deletion of mTOR has protected against post-traumatic osteoarthritis (OA) in male mice, however, effects of pharmacological mTOR-inhibition are equivocal and have not been tested in aging models nor in female subjects. Therefore, the goal of this study was to determine if mTOR-inhibition by rapamycin can modify OA pathology in aging non-human primates and female mice. MethodsCommon marmosets were administered oral rapamycin (1mg/kg/day) or vehicle starting near mid-life until death. Five-month-old, female C57BL/6J mice were treated with vehicle or rapamycin (IP, 2mg/kg, 3x/week) for 8-weeks following non-invasive ACL rupture. Knee OA pathology was assessed via microCT and histology. Phosphorylation of mTORC1 (p-RPS6S235/36) and mTORC2 (p-AktS473, p-NDRG1T638, p-PKCT348) substrates were evaluated via western blot in articular cartilage, meniscus, and/or infrapatellar fat pad. ATDC5 cells were cultured with rapamycin to determine time and dose effects on mTORC1/2 signaling. ResultsIn marmosets, rapamycin did not impact age-related radiographic OA severity or cartilage pathology but increased medial meniscus calcification and lowered lateral tibia subchondral thickness, particularly in females. In female mice, rapamycin worsened ACLR-induced meniscus calcification and cartilage pathology. In marmoset and mouse joint tissues, rapamycin inhibited mTORC1 and increased p-AktS473 but not p-NDRG1T638 or p-PKCT348. This mTOR signaling pattern was replicated in ATDC5 cells during exposure to low concentrations of rapamycin. ConclusionsRapamycin attenuated mTORC1 signaling with feedback activation of AktS473 in articular cartilage, meniscus, and/or infrapatellar fat pad and was accompanied by deleterious effects on meniscus calcification and/or cartilage pathology in female mice and common marmosets.

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BibTeXRIS

Minton, D. M., Ailiani, A. R., Focht, M. D. K., Kersh, M. E., Marolf, A. J., Santangelo, K. S., Salmon, A. B., Konopka, A. R.. 2024-05-17. Impact of long-term rapamycin treatment on age-related osteoarthritis in common marmoset. https://doi.org/10.1101/2024.05.14.594256

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