Gradual labeling with fluorogenic probes for MINFLUX nanoscopy of densely packed structures

yao, l.; Si, D.; Chen, L.; Li, S.; Guan, J.; Ma, J.; zhang, q.; wang, j.; wang, l.; Gu, M.

doi:10.1101/2024.05.08.593253

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bioRxiv · 10.1101/2024.05.08.593253

Gradual labeling with fluorogenic probes for MINFLUX nanoscopy of densely packed structures

yao, l.·Si, D.·Chen, L.·Li, S.·Guan, J.·Ma, J.·zhang, q.·wang, j.·wang, l.·Gu, M.

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Abstract

Single-molecule localization in densely packed structures represents a formidable challenge in nanoscopy. Here we develop a gradual labeling method with fluorogenic probes for MINFLUX nanoscopy (GLF-MINFLUX), with which individual proteins in packed structures can be sequential illuminated and quantitatively localized at the nanoscale solely by adjusting probe concentration. With a 1.7-fold localization precision improvement and 2.2-fold faster acquisition, GLF-MINFLUX enables dual-channel nanoscale imaging of densely neuronal microtubules and microfilaments, and three-dimensional quantitative mapping of individual TOM20 proteins in mitochondrial clusters.

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yao, l., Si, D., Chen, L., Li, S., Guan, J., Ma, J., zhang, q., wang, j., wang, l., Gu, M.. 2024-05-11. Gradual labeling with fluorogenic probes for MINFLUX nanoscopy of densely packed structures. https://doi.org/10.1101/2024.05.08.593253

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