Search bioRxiv⌕ Search
Home/Archive/bioRxiv

bioRxiv · 10.1101/2024.05.02.592171

Contrasting patterns of spatial genetic structure in endangered southern damselfly (Coenagrion mercuriale) populations facing habitat fragmentation and urbanisation.

Leveque, A.·Duputie, A.·Vignon, V.·Duez, F.·Gode, C.·Vanappelghem, C.·Arnaud, J.-F.
View original source ↗Export metadata ↓

Abstract

AimHuman-induced environmental changes result in habitat loss and fragmentation, impacting wildlife population genetic structure and evolution. Urbanised and geographically peripheral areas often represent unfavourable environments, reducing connectivity among populations and causing higher population genetic differentiation and lower intra-population genetic diversity. We examined how geographic peripherality and anthropogenic pressures affect genetic diversity and genetic differentiation in the protected southern damselfly (Coenagrion mercuriale, Odonata), which has low dispersal capabilities and specific habitat requirements and whose populations are declining. LocationWe studied two areas: one in semi-natural habitats at the periphery of the species geographic range (northern France) and the other more central to the species range, in an urbanised area surrounding the city of Strasbourg (Alsace, eastern France). MethodsWe genotyped 2743 individuals from 128 populations using eleven microsatellite loci. We analysed the spatial distribution of neutral genetic diversity (allelic richness, heterozygosity, levels of inbreeding, genetic relatedness), the extent of genetic differentiation, and population affiliations (sPCA analyses) within the two areas. We also examined fine-scale patterns of gene flow in the urbanised area of Alsace by investigating patterns of isolation by distance and estimating effective migration surfaces (EEMS method). ResultsNorthern peripheral populations showed lower levels of genetic diversity and higher levels of genetic differentiation than central Alsacian populations. Although located in anthropised habitats, geographically central Alsacian populations showed high levels of gene flow, with dispersal events mainly occurring overland and not restricted to watercourses. However, the highly urbanised city of Strasbourg negatively impacted nearby populations by reducing levels of genetic diversity and increasing population genetic differentiation. Main conclusionsThese results showed the need for management action by restoring breeding sites and creating migratory corridors for peripheral southern damselfly populations. However, our results also highlighted the resilience of southern damselfly in central range populations facing strong urbanisation pressures.

Source connections

Explore related subjects

evolutionary biologyPreprint

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Leveque, A., Duputie, A., Vignon, V., Duez, F., Gode, C., Vanappelghem, C., Arnaud, J.-F.. 2024-05-05. Contrasting patterns of spatial genetic structure in endangered southern damselfly (Coenagrion mercuriale) populations facing habitat fragmentation and urbanisation.. https://doi.org/10.1101/2024.05.02.592171

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related preprints

bioRxiv2026

Measuring the strength of selection and pollen limitation on flower colour along an urban rural cline in Hesperis matronalis

Closely located urban and rural environments can differ in temperature, water availability, species diversity, and more. As a result, organisms in urban environments may experience different selection than those in rural environments. We investigated whether differences in selection might explain a previously identified urban-rural cline in the flower colour of Hesperis matronalis (dame's rocket). To explore whether plants in urban and rural environments are impacted by possible differences in pollinator availability or behavior, we performed a pollen supplementation experiment. We did not detect an effect of pollen supplementation on seed production in either setting (urban or rural), indicating that there is limited opportunity for any differences in pollinator availability or visitation to explain the cline in flower colour. We also measured the strength of selection acting on flower colour to investigate if it differed between urban and rural populations. In all but one population, we failed to detect significant linear or non-linear selection on flower colour, suggesting that selection on flower colour does not differ between urban and rural populations. Our results suggest that the urban-rural flower colour cline is likely not caused by differences in selection or pollen-limitation and may be due to stochastic forces.

evolutionary biology↗
bioRxiv2026

An elongated lifespan is associated with higher oxidative protection of flight tissue in Heliconiini butterflies

Evolutionary theories of ageing often invoke trade-offs in resource allocation between somatic maintenance and other important physiological functions. One proposed mechanistic mediator of these trade-offs is oxidative stress, which arises from an imbalance between an organism's production of reactive oxygen species and its ability to detoxify them. Accordingly, many long-lived species across the animal kingdom show evidence for resistance to oxidative stress. However, it can be difficult to associate lifespan differences with oxidative phenotypes when comparing species across wide taxonomic distances, which may also show large differences in physiology. Butterflies in the long-lived Heliconius genus show a 3-fold lifespan extension and slowed ageing in comparison with their close relatives in the Heliconiini tribe, to whom they are phylogenetically, ecologically, and physiologically similar. However, proximate explanations for this extended longevity are currently lacking. Here, we leverage the neat comparative framework between Heliconius hecale and Dryas iulia, a shorter-lived Heliconiini outgroup, to test whether the observed lifespan differences between the two species are associated with differences in oxidative physiology. Our findings suggest superior oxidative protection of thoracic tissue in the longer-lived H. hecale compared to D. iulia, as evidenced by increased superoxide dismutase activity, increased total antioxidant capacity, and a corresponding reduction in lipid peroxidation in this tissue. Considering the metabolic demands of insect flight, we suggest that this oxidative phenotype may contribute to the extended longevity of Heliconius by enhancing protection of thoracic tissues against oxidative damage, representing a plausible proximate pro-longevity mechanism in this genus.

evolutionary biology↗
bioRxiv2026

Both genetic and social inheritance influence female aggressive and submissive behavior in a wild primate

Agonistic interactions are important determinants of resource access in wild animals, but their genetic basis remains poorly understood. Here we use a quantitative genetics approach to estimate the heritability of aggressive and submissive behavior of wild female baboons, considering relative rates of both individual and dyadic aggression and submission. To control for the social inheritance that creates kin-based dominance hierarchies, we implemented a multi-matrix model that included a social status similarity matrix alongside the additive genetic matrix. Despite a substantial contribution of social inheritance, especially for submissive behavior, we found heritable components for both aggressive and submissive behavior (h2=0.21 and h2=0.09-0.11, respectively). In dyadic models, aggressive behavior was primarily influenced by the aggressor's genotype (a direct genetic effect), while submissive behavior was primarily affected by the social partner's genotype (an indirect genetic effect). Finally, we found evidence for a negative genetic (but not phenotypic) correlation between aggressive and submissive behavior within dyads, and a negative genetic correlation between submissive behavior and eliciting submissive behavior. Together, our results highlight how direct genetic, indirect genetic, and social inheritance effects combine to influence primate social behavior.

evolutionary biology↗