Electrophysiological responses to appetitive and consummatory behavior in the rostral nucleus tractus solitarius in awake, unrestrained rats

Abstract

As the intermediate nucleus in the brainstem receiving information from the tongue and transmitting information upstream, the rostral portion of the nucleus tractus solitarius (rNTS) is most often described as a "taste relay". Although recent evidence implicates the NTS in a broad neural circuit involved in regulating ingestion, there is little information about how cells in this structure respond when an animal is eating solid food. Here, single cells in the rNTS were recorded in awake, unrestrained rats as they explored and ate solid foods (Eating paradigm) chosen to correspond to the basic taste qualities: milk chocolate for sweet, salted peanuts for salty, Granny Smith apples for sour and broccoli for bitter. A subset of cells was also recorded as the animal licked exemplars of the five basic taste qualities: sucrose, NaCl, citric acid, quinine and MSG (Lick paradigm). Results showed that most cells were excited by exploration of a food-filled well, sometimes responding prior to contact with the food. In contrast, cells that were excited by food well exploration became significantly less active while the animal was eating the food. Most cells were broadly tuned across foods, and those cells that were recorded in both the Lick and Eating paradigms showed little correspondence in their tuning across paradigms. The preponderance of robust responses to the appetitive versus the consummatory phase of ingestion suggests that multimodal convergence onto cells in the rNTS may be used in decision making about ingestion. Significance StatementThe rostral part of the NTS has traditionally, but perhaps narrowly, been thought of as a "taste relay". While it is true that this structure receives and transmits information about tastants in the mouth to higher order structures in the central gustatory pathway, data presented here show that its function is more diverse. Specifically, in addition to the responses to liquid tastants in the mouth, its responses to approach and exploration of solid food define a significant role for the rNTS in the appetitive phase of eating. Moreover, responses to food consumption, albeit weaker than those during appetitive behavior, buttress the idea that the rNTS is part of the larger hindbrain circuit guiding energy regulation.