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bioRxiv · 10.1101/2024.04.28.591475

Creating coveted bioluminescence colors for simultaneous myriad-color bioimaging

Hattori, M.·Wazawa, T.·Orioka, M.·Hiruta, Y.·Nagai, T.
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Abstract

Bioluminescence, an optical marker that does not require excitation by light, allows researchers to simultaneously observe multiple targets, each exhibiting a different color. Notably, the colors of the bioluminescent proteins must sufficiently vary to enable simultaneous detection. Here, we aimed to introduce a method that can be used to expand the color variation by tuning dual-acceptor bioluminescence resonance energy transfer (BRET). Using this approach, we could visualize multiple targets with up to 20 colors through single-shot acquisition using a color CMOS camera. Overall, this method enables simple and simultaneous observation of numerous biological targets and phenomena.

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Hattori, M., Wazawa, T., Orioka, M., Hiruta, Y., Nagai, T.. 2024-04-30. Creating coveted bioluminescence colors for simultaneous myriad-color bioimaging. https://doi.org/10.1101/2024.04.28.591475

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