Biochemical and structural insights into a 5' to 3' RNA ligase reveal a potential role in tRNA ligation

Hu, Y.; Lopez, V. A.; Xu, H.; Pfister, J. P.; Song, B.; Servage, K. A.; Sakurai, M.; Jones, B. T.; Mendell, J. T.; Wang, T.; Wu, J.; Lambowitz, A. M.; Tomchick, D. R.; Pawlowski, K.; Tagliabracci, V. S.

doi:10.1101/2024.04.24.590974

bioRxiv · 10.1101/2024.04.24.590974

Biochemical and structural insights into a 5' to 3' RNA ligase reveal a potential role in tRNA ligation

Hu, Y.·Lopez, V. A.·Xu, H.·Pfister, J. P.·Song, B.·Servage, K. A.·Sakurai, M.·Jones, B. T.·Mendell, J. T.·Wang, T.·Wu, J.·Lambowitz, A. M.·Tomchick, D. R.·Pawlowski, K.·Tagliabracci, V. S.

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Abstract

ATP-grasp superfamily enzymes contain a hand-like ATP-binding fold and catalyze a variety of reactions using a similar catalytic mechanism. More than 30 protein families are categorized in this superfamily, and they are involved in a plethora of cellular processes and human diseases. Here we identify C12orf29 as an atypical ATP-grasp enzyme that ligates RNA. Human C12orf29 and its homologs auto-adenylate on an active site Lys residue as part of a reaction intermediate that specifically ligates RNA halves containing a 5-phosphate and a 3-hydroxyl. C12orf29 binds tRNA in cells and can ligate tRNA within the anticodon loop in vitro. Genetic depletion of c12orf29 in female mice alters global tRNA levels in brain. Furthermore, crystal structures of a C12orf29 homolog from Yasminevirus bound to nucleotides reveal a minimal and atypical RNA ligase fold with a unique active site architecture that participates in catalysis. Collectively, our results identify C12orf29 as an RNA ligase and suggest its involvement in tRNA biology. Significance StatementATP-grasp enzymes share an atypical ATP-binding fold and catalyze a diverse set of reactions involved in many essential cellular processes. We identified C12orf29 as an atypical ATP-grasp enzyme. Our biochemical and structural characterizations reveal this enzyme to be a 5 to 3 RNA ligase, structurally and functionally similar to the phage T4 RNA ligase. C12orf29 can ligate tRNAs in vitro and C12orf29 knockout female mice have altered tRNA levels in brain. We also report structures of C12orf29, which have revealed critical insights into the mode of ATP binding and catalysis. Our work suggests that C12orf29 may be a new player in the regulation of tRNAs.

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Hu, Y., Lopez, V. A., Xu, H., Pfister, J. P., Song, B., Servage, K. A., Sakurai, M., Jones, B. T., Mendell, J. T., Wang, T., Wu, J., Lambowitz, A. M., Tomchick, D. R., Pawlowski, K., Tagliabracci, V. S.. 2024-04-24. Biochemical and structural insights into a 5' to 3' RNA ligase reveal a potential role in tRNA ligation. https://doi.org/10.1101/2024.04.24.590974

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