Alternative oxidase alleviates mitochondrial oxidative stress during limited nitrate reduction in Arabidopsis thaliana

Abstract

The conversion of nitrate to ammonium, i.e., nitrate reduction, is a major consumer of reductants in plants. Previous studies have reported that the mitochondrial alternative oxidase (AOX) is upregulated under limited nitrate reduction conditions, including no/low nitrate or when ammonium is the sole nitrogen (N) source. Electron transfer from ubiquinone to AOX bypasses the proton-pumping complexes III and IV, thereby consuming reductants efficiently. Thus, upregulated AOX under limited nitrate reduction may dissipate excessive reductants and thereby attenuate oxidative stress. Nevertheless, so far there is no firm evidence for this hypothesis due to the lack of experimental systems to analyze the direct relationship between nitrate reduction and AOX. We therefore developed a novel culturing system for A. thaliana that manipulates activities of nitrate reduction and AOX separately without causing N starvation, ammonium toxicity, and lack of nitrate signal. Using this system we examined genome-wide gene expression and plant growth to better understand the relationship between AOX and nitrate reduction. Here we show that AOX alleviates mitochondrial oxidative stress and sustains plant growth under limited nitrate reduction.