Satellite glial cells from adult DRG dedifferentiate in vitro and can be reprogrammed into nociceptor-like neurons

Sodmann, A.; Koehler, N.; Esfahani, N. M.; Schuhkraft, N.; Aue, A.; Jager, S. E.; Bischler, T.; Imdahl, F.; Graefenhan, T.; Leipold, E.; Rittner, H. L.; Blum, R.

doi:10.1101/2024.04.09.588701

bioRxiv · 10.1101/2024.04.09.588701

Satellite glial cells from adult DRG dedifferentiate in vitro and can be reprogrammed into nociceptor-like neurons

Sodmann, A.·Koehler, N.·Esfahani, N. M.·Schuhkraft, N.·Aue, A.·Jager, S. E.·Bischler, T.·Imdahl, F.·Graefenhan, T.·Leipold, E.·Rittner, H. L.·Blum, R.

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Abstract

In dorsal root ganglia (DRG), neuronal loss has been reported in patients with neuropathic pain, raising the question of whether the DRG, as part of the peripheral nervous system (PNS), harbor an endogenous cell source for neural repair. We found that adult mouse DRG harbor glial cells that dedifferentiate in vitro into Sox2/Sox10-positive glial progenitor-like cells. Coexpression of the developmental transcription factors Neurog1 and Neurog2 was sufficient to induce both neuronal and glial phenotypes. Nerve growth factor supported the maturation of a subset of neurons into nociceptor-like cells expressing functional TrpA1, TrpV1, and TTX-resistant NaV channels. We report the limitation that we miss factors allowing consistent maturation to the sensory neuron profile. In summary, in the PNS, adult DRG-derived glial cells can acquire neural progenitor-like properties, show bipotent reprogramming competence, and may serve as an intrinsic cell source for sensory circuit regeneration.

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Sodmann, A., Koehler, N., Esfahani, N. M., Schuhkraft, N., Aue, A., Jager, S. E., Bischler, T., Imdahl, F., Graefenhan, T., Leipold, E., Rittner, H. L., Blum, R.. 2024-04-12. Satellite glial cells from adult DRG dedifferentiate in vitro and can be reprogrammed into nociceptor-like neurons. https://doi.org/10.1101/2024.04.09.588701

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