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bioRxiv · 10.1101/2024.04.08.588545

Physiological and transcriptional changes associated with obligate aestivation in the cabbage stem flea beetle (Psylliodes chrysocephala)

Guney, G.·Cedden, D.·Koernig, J.·Ulber, B.·Beran, F.·Scholten, S.·Rostas, M.
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Abstract

1Aestivation is a form of seasonal dormancy observed in various insect species, usually coinciding with the summer season. Psylliodes chrysocephala (Coleoptera: Chrysomelidae), the cabbage stem flea beetle, is a key pest of oilseed rape and obligatorily aestivates as adult in late summer. At present, our understanding of the physiological and transcriptional changes linked to aestivation in P. chrysocephala is still limited. In this study, physiological parameters and RNA-seq analyses were performed with laboratory-reared beetles at pre-aestivation, aestivation, and post-aestivation stages. Measurements of CO2 production supported the notion that aestivating beetles dramatically reduce their metabolic rate and, together with assessments of reproductive maturation, allowed precise discrimination between the three adult stages. Aestivating beetles showed a reduction in carbohydrate reserves and an increase in lipid reserves compared to pre-aestivating beetles, indicating that aestivation is associated with drastic changes in energy metabolism. In agreement with these findings, we found that genes involved in carbohydrate and lipid metabolism, digestion, and mitochondrial activity are differentially expressed between the three stages. Furthermore, RNA-seq analysis suggested the regulation of transcription factors associated with aestivation maintenance and the involvement of cytochrome P450s in conferring a summer-resistant phenotype during the aestivation period. In conclusion, this study represents the first exploration of the transcriptomic and physiological aspects of the aestivation response in P. chrysocephala. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=109 SRC="FIGDIR/small/588545v1_ufig1.gif" ALT="Figure 1"> View larger version (31K): org.highwire.dtl.DTLVardef@10d486forg.highwire.dtl.DTLVardef@10e9e1corg.highwire.dtl.DTLVardef@ae63aforg.highwire.dtl.DTLVardef@112985c_HPS_FORMAT_FIGEXP M_FIG C_FIG O_LIP. chrysocephala obligatorily aestivate as sexually immature adults in summer C_LIO_LIAestivation entails metabolic suppression, body composition changes and resistance C_LIO_LIAccordingly, metabolism and stress genes were differentially expressed C_LIO_LIThe findings can support the development of innovative pest management strategies C_LI

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Guney, G., Cedden, D., Koernig, J., Ulber, B., Beran, F., Scholten, S., Rostas, M.. 2024-04-11. Physiological and transcriptional changes associated with obligate aestivation in the cabbage stem flea beetle (Psylliodes chrysocephala). https://doi.org/10.1101/2024.04.08.588545

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