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bioRxiv · 10.1101/2024.03.28.587148

Transcriptomic analysis of the juvenile to adult transition in the mouse corpus callosum

Hoshino, T.·Takase, H.·Hamanaka, G.·Kimura, S.·Fukuda, N.·Mandeville, E. T.·Lok, J.·Lo, E. H.·Arai, K.
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Abstract

The corpus callosum, a major white matter tract in the brain, undergoes age-related functional changes. To extend our investigation of age-related gene expression dynamics in the mouse corpus callosum, we compared RNA-seq data from 2-week-old and 12-week-old wild-type C57BL/6J mice and identified the differentially expressed genes (e.g., Serpinb1a, Ndrg1, Dnmt3a, etc.) between these ages. Interestingly, we found that genes highly expressed in myelinating oligodendrocytes were upregulated in 12-week-old mice compared to 2-week-old mice, while genes highly expressed in oligodendrocyte precursor cells (OPCs) and newly formed oligodendrocytes were downregulated. Furthermore, by comparing these genes with the datasets from 20-week-old and 96-week-old mice, we identified novel sets of genes with age-dependent variations in the corpus callosum. These gene expression changes potentially affect key biological pathways and may be closely linked to age-related neurological disorders, including dementia and stroke. Therefore, our results provide an additional dataset to explore age-dependent gene expression dynamics of oligodendrocyte lineage cells in the corpus callosum.

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Hoshino, T., Takase, H., Hamanaka, G., Kimura, S., Fukuda, N., Mandeville, E. T., Lok, J., Lo, E. H., Arai, K.. 2024-03-30. Transcriptomic analysis of the juvenile to adult transition in the mouse corpus callosum. https://doi.org/10.1101/2024.03.28.587148

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