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bioRxiv · 10.1101/2024.03.26.586884

The Scribble/SGEF/Dlg1 complex regulates the stability of apical junctions in epithelial cells

Abstract

SGEF, a RhoG specific GEF, can form a ternary complex with the Scribble polarity complex proteins Scribble and Dlg1, which regulates the formation and maintenance of adherens junctions and barrier function of epithelial cells. Notably, silencing SGEF results in a dramatic downregulation of the expression of both E-cadherin and ZO-1. However, the molecular mechanisms involved in the regulation of this pathway are not known. Here, we describe a novel signaling pathway governed by the Scribble/SGEF/Dlg1 complex. Our results show that an intact ternary complex is required to maintain the stability of the apical junctions, the expression of ZO-1, and TJ permeability. In contrast, only SGEF is necessary to regulate E-cadherin expression. The absence of SGEF destabilizes the E-cadherin/catenin complex at the membrane, triggering a positive feedback loop that exacerbates the phenotype through the repression of E-cadherin transcription in a process that involves the internalization of E-cadherin by endocytosis, {beta}-catenin signaling and the transcriptional repressor Slug.

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BibTeXRIS

Rabino, A. F., Awadia, S., Ali, N. N., Edson, A. L., Garcia-Mata, R.. 2024-03-27. The Scribble/SGEF/Dlg1 complex regulates the stability of apical junctions in epithelial cells. https://doi.org/10.1101/2024.03.26.586884

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