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bioRxiv · 10.1101/2024.03.20.586023

pSPObooster: a plasmid system to improve sporulation efficiency of Saccharomyces cerevisiae lab strains

Loll-Krippleber, R.·Jiang, Y. K.·Brown, G. W.
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Abstract

Common S. cerevisiae lab yeast strains derived from S288C have meiotic defects and therefore are poor sporulators. Here, we developed a plasmid system containing corrected alleles of the MKT1 and RME1 genes to rescue the meiotic defects and show that standard BY4741 and BY4742 strains containing the plasmid display faster and more efficient sporulation. The plasmid, pSPObooster, can be maintained as an episome and easily cured or stably integrated into the genome at a single locus. We demonstrate the use of pSPObooster in low- and high-throughput yeast genetic manipulations and show that it can expedite both procedures without impacting strain behavior. Take AwayO_LIpSPObooster contains corrected alleles or RME1 and MKT1. C_LIO_LIpSPObooster can be maintained as an episome or integrated. C_LIO_LIpSPObooster increases sporulation efficiency by up to 13-fold. C_LIO_LIpSPObooster can be used to speed up high-throughput yeast strain engineering. C_LI

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Loll-Krippleber, R., Jiang, Y. K., Brown, G. W.. 2024-03-20. pSPObooster: a plasmid system to improve sporulation efficiency of Saccharomyces cerevisiae lab strains. https://doi.org/10.1101/2024.03.20.586023

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