Forelimb movements contribute to hindlimb cutaneous reflexes during locomotion in cats

Harnie, J.; Al Arab, R.; Mari, S.; Yassine, S.; Eddaoui, O.; Jehannin, P.; Audet, J.; Lecomte, C. G.; Iorio-Morin, C.; Prilutsky, B. I.; Rybak, I. A.; Frigon, A.

doi:10.1101/2024.03.13.584812

bioRxiv · 10.1101/2024.03.13.584812

Forelimb movements contribute to hindlimb cutaneous reflexes during locomotion in cats

Harnie, J.·Al Arab, R.·Mari, S.·Yassine, S.·Eddaoui, O.·Jehannin, P.·Audet, J.·Lecomte, C. G.·Iorio-Morin, C.·Prilutsky, B. I.·Rybak, I. A.·Frigon, A.

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Abstract

During quadrupedal locomotion, central circuits interacting with somatosensory feedback coordinate forelimb and hindlimb movements. How this is achieved is not clear. To determine if forelimb movements modulate hindlimb cutaneous reflexes involved in responding to an external perturbation, we stimulated the superficial peroneal nerve in six intact cats during quadrupedal locomotion and during hindlimb-only locomotion (with forelimbs standing on stationary platform) and in two spinal-transected cats during hindlimb-only locomotion. We compared cutaneous reflexes evoked in six ipsilateral and four contralateral hindlimb muscles. Results showed similar occurrence and phase-dependent modulation of short-latency inhibitory and excitatory responses during quadrupedal and hindlimb-only locomotion in intact cats. However, the depth of modulation was reduced in the ipsilateral semitendinosus during hindlimb-only locomotion. Additionally, longer-latency responses occurred less frequently in extensor muscles bilaterally during hindlimb-only locomotion while short-latency inhibitory and longer-latency excitatory responses occurred more frequently in the ipsilateral and contralateral sartorius anterior, respectively. After spinal transection, short-latency inhibitory and excitatory responses were similar to both intact conditions, while mid- or longer-excitatory responses were reduced or abolished. Our results suggest that the absence of forelimb movements suppresses inputs from supraspinal structures and/or cervical cord that normally contribute to longer-latency reflex responses in hindlimb extensor muscles. NEW & NOTEWORTHYDuring quadrupedal locomotion, the coordination of forelimb and hindlimb movements involves central circuits and somatosensory feedback. To demonstrate how forelimb movement affects hindlimb cutaneous reflexes during locomotion, we stimulated the superficial peroneal nerve in intact cats during quadrupedal and hindlimb-only locomotion, as well as in spinal-transected cats during hindlimb-only locomotion. We show that forelimb movement influences the modulation of hindlimb cutaneous reflexes, particularly the occurrence of long-latency reflex responses.

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Harnie, J., Al Arab, R., Mari, S., Yassine, S., Eddaoui, O., Jehannin, P., Audet, J., Lecomte, C. G., Iorio-Morin, C., Prilutsky, B. I., Rybak, I. A., Frigon, A.. 2024-03-14. Forelimb movements contribute to hindlimb cutaneous reflexes during locomotion in cats. https://doi.org/10.1101/2024.03.13.584812

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