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bioRxiv · 10.1101/2024.03.12.584205

NEMO: Improved and accurate models for identification of 6mA using Nanopore sequencing

Kulkarni, O. V.·Zaveri, L.·Singh, N. K.·Mathew, R. J.·Ara, S.·Garde, S.·Reddy, M.·Tallapaka, K. B.·Sowpati, D. T.
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Abstract

DNA methylation plays a key role in epigenetic regulation across lifeforms. Nanopore sequencing enables direct detection of base modifications. While multiple tools are currently available for studying 5-methylcytosine (5mC), there is a paucity of models that can detect 6-methyladenine (6mA) from raw nanopore data. Leveraging the motif-driven nature of bacterial methylation systems, we generated 6mA identification models that vastly surpass the accuracy of the current best model. Our work enables the study of 6mA at a single-base resolution in new as well as existing nanopore datasets.

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Kulkarni, O. V., Zaveri, L., Singh, N. K., Mathew, R. J., Ara, S., Garde, S., Reddy, M., Tallapaka, K. B., Sowpati, D. T.. 2024-03-13. NEMO: Improved and accurate models for identification of 6mA using Nanopore sequencing. https://doi.org/10.1101/2024.03.12.584205

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