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bioRxiv · 10.1101/2024.03.04.583254

Subtomogram averaging of helical filaments in cryo-electron tomography

Zhang, X.·Mahamid, J.
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Abstract

Helical filaments are essential macromolecular elements in cellular organization and dynamics. Recent advances in cryo-electron tomography allow faithful imaging of isolated or in-cell helical filaments. Here, we present a protocol to generate density maps at sub-nanometer resolution of helical filaments by subtomogram averaging, exemplified with isolated mumps virus nucleocapsids and their in-cell form as an extension of the method. We detail procedures from pre-processing of tilt series frames to refinement of reconstructed averages for streamlined data processing of helical filaments.

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Zhang, X., Mahamid, J.. 2024-03-06. Subtomogram averaging of helical filaments in cryo-electron tomography. https://doi.org/10.1101/2024.03.04.583254

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