A series of spontaneously blinking dyes for super-resolution microscopy

Holland, K. L.; Plutkis, S. E.; Daugird, T. A.; Sau, A.; Grimm, J. B.; English, B. P.; Zheng, Q.; Dave, S.; Rahman, F.; Xie, L.; Dong, P.; Tkachuk, A. N.; Brown, T. A.; Singer, R. H.; Liu, Z. J.; Galbraith, C. G.; Musser, S. M.; Legant, W. R.; Lavis, L. D.

doi:10.1101/2024.02.23.581625

bioRxiv · 10.1101/2024.02.23.581625

A series of spontaneously blinking dyes for super-resolution microscopy

Holland, K. L.·Plutkis, S. E.·Daugird, T. A.·Sau, A.·Grimm, J. B.·English, B. P.·Zheng, Q.·Dave, S.·Rahman, F.·Xie, L.·Dong, P.·Tkachuk, A. N.·Brown, T. A.·Singer, R. H.·Liu, Z. J.·Galbraith, C. G.·Musser, S. M.·Legant, W. R.·Lavis, L. D.

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Abstract

Spontaneously blinking fluorophores permit the detection and localization of individual molecules without reducing buffers or caging groups, thus simplifying single-molecule localization microscopy (SMLM). The intrinsic blinking properties of such dyes are dictated by molecular structure and modulated by environment, which can limit utility. We report a series of tuned spontaneously blinking dyes with duty cycles that span two orders of magnitude, allowing facile SMLM in cells and dense biomolecular structures.

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Holland, K. L., Plutkis, S. E., Daugird, T. A., Sau, A., Grimm, J. B., English, B. P., Zheng, Q., Dave, S., Rahman, F., Xie, L., Dong, P., Tkachuk, A. N., Brown, T. A., Singer, R. H., Liu, Z. J., Galbraith, C. G., Musser, S. M., Legant, W. R., Lavis, L. D.. 2024-02-24. A series of spontaneously blinking dyes for super-resolution microscopy. https://doi.org/10.1101/2024.02.23.581625

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