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bioRxiv · 10.1101/2024.02.19.577657

Journey to the center of the phage; revealing the ejectosome of Pectobacterium bacteriophage {Phi}M1

Eruera, A.-R.·Hodgkinson-Bean, J.·Rutter, G. L.·Hills, F. R.·Kumaran, R.·Crowe, A. J. M.·Jadav, N.·McJarrow-Keller, K. L.·Jorge, F. E.·Hyun, J.·Kim, H.·Ryu, B.·Bostina, M.
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Abstract

Podophages that infect gram-negative bacteria, such as Pectobacterium pathogen {Phi}M1, encode tail assemblies too short to extend across the complex gram-negative cell wall. To overcome this, podophages encode a large protein complex (ejectosome) packaged inside the viral capsid and correspondingly ejected during infection to form a transient channel that spans the periplasmic space. Here we describe the ejectosome of bacteriophage {Phi}M1 to a resolution of 3.32 [A] by single particle cryo-EM. The core consists of tetrameric and octameric ejection proteins which form a [~]1.5 MDa ejectosome that must transition through the [~]30 [A] aperture created by the short tail nozzle assembly that acts as the conduit for the passage of DNA during infection. The ejectosome forms several grooves into which coils of genomic DNA are fit before the DNA sharply turns and goes down the tunnel and into the portal. In addition, we reconstructed the icosahedral capsid and hybrid tail apparatus to resolutions between 3.04 [A] and 3.23 [A], and note an uncommon fold adopted by the dimerized decoration proteins which further emphasize the structural diversity of podophages. These reconstructions have allowed the generation of a complete atomic model of the {Phi}M1, uncovering two distinct decoration proteins and highlighting the exquisite structural diversity of tailed bacteriophages. Significance StatementThis study resolves the cryo-EM structure of bacteriophage {Phi}M1, which possesses several unique and interesting structural elements, including a pair of distinct decoration proteins that are underreported in tailed DNA phages. Significantly, we also report the internal ejectosome proteins of {Phi}M1, which are highly non-conserved with previously solved proteins to date and demonstrate the structural diversity of ejection proteins. The ejectosome reveals a DNA spooling phenomenon whereby the viral genome wraps around the ejectosome within the capsid, which has never been reported before. We provide a clear, step-by-step method for the technically challenging reconstruction of ejectosomes using standard, open-source software.

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Eruera, A.-R., Hodgkinson-Bean, J., Rutter, G. L., Hills, F. R., Kumaran, R., Crowe, A. J. M., Jadav, N., McJarrow-Keller, K. L., Jorge, F. E., Hyun, J., Kim, H., Ryu, B., Bostina, M.. 2024-02-19. Journey to the center of the phage; revealing the ejectosome of Pectobacterium bacteriophage {Phi}M1. https://doi.org/10.1101/2024.02.19.577657

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