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bioRxiv · 10.1101/2024.02.14.580305

Paired metagenomic and chemical evaluation of an aflatoxin contaminated dog kibble

Abstract

Identification of chemical toxins from complex or highly processed foods can present needle in the haystack challenges for chemists. Metagenomic data can guide chemical toxicity evaluations with DNA-based description of the wholistic composition (bacterial, eukaryotic, protozoal, viral, and antimicrobial resistance) of any food suspected to harbor toxins, allergens, or pathogens. This approach can focus chemistry-based diagnostics, improve risk assessment, and address data gaps. There is increasing recognition that simultaneously co-occurring mycotoxins, either from single or multiple species, can impact dietary toxicity. Here we evaluate an aflatoxin contaminated kibble with known levels of specific mycotoxins and demonstrate that the abundance of DNA from putative aflatoxigenic Aspergillus spp. correlated with levels of aflatoxin quantified by Liquid Chromatography Mass Spectrometry (LCMS). Metagenomic data also identified an expansive range of co-occurring fungal taxa which may produce additional mycotoxins. Metagenomic data paired with chemical data provides a novel modality to address current data gaps pertaining to mycotoxin toxicity exposures, toxigenic fungal taxonomy, and mycotoxins of emerging concern.

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BibTeXRIS

Ottesen, A., Kocurek, B. J., Reed, E., Commichaux, S., Mammel, M. K., Ramachandran, P., McDermott, P. F., Flannery, B. M., Strain, E. A.. 2024-02-14. Paired metagenomic and chemical evaluation of an aflatoxin contaminated dog kibble. https://doi.org/10.1101/2024.02.14.580305

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