High-throughput nano-flow proteomics using a dual-column electrospray source

Staes, A.; Boucher, K.; Dufour, S.; Maia, T. M.; Timmerman, E.; Van Haver, D.; Pauwels, J.; Demol, H.; Vandenbussche, J.; Impens, F.; Devos, S.

doi:10.1101/2024.02.13.580084

bioRxiv · 10.1101/2024.02.13.580084

High-throughput nano-flow proteomics using a dual-column electrospray source

Staes, A.·Boucher, K.·Dufour, S.·Maia, T. M.·Timmerman, E.·Van Haver, D.·Pauwels, J.·Demol, H.·Vandenbussche, J.·Impens, F.·Devos, S.

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Abstract

With current trends in proteomics, especially regarding clinical and low input (to single cell) samples, it is increasingly important to both maximize the throughput of the analysis and maintain as much sensitivity as possible. The new generation of mass spectrometers (MS) are taking a huge leap in sensitivity, allowing to analyze samples with shorter liquid chromatography (LC) methods while digging as deep in the proteome. However, the throughput can be doubled by implementing a dual column nano-LC-MS configuration. For this purpose, we used a dual-column setup with a two-outlet electrospray source, and compared it to a classic dual-column setup with a single-outlet source.

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Staes, A., Boucher, K., Dufour, S., Maia, T. M., Timmerman, E., Van Haver, D., Pauwels, J., Demol, H., Vandenbussche, J., Impens, F., Devos, S.. 2024-02-13. High-throughput nano-flow proteomics using a dual-column electrospray source. https://doi.org/10.1101/2024.02.13.580084

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