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bioRxiv · 10.1101/2024.02.13.580069

Do light eaters live shorter lives? The case of ultralight Caenorhabditis elegans

Zhang, X.·Gharibi, H.·Beusch, C. M.·Meng, Z.·Saei Dibavar, A.·Gaetani, M.·Zubarev, R.
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Abstract

The idea that ingesting heavy stable isotopes can increase longevity emerged shortly after the discovery of deuterium in the early 1930s and has been extensively tested since then on animals. Here we present the first experimental evidence for the opposite. Growing C. elegans on bacteria E. coli that are in turn fed on a diet depleted of heavy isotopes of C, H, N and O produced ultralight worms that grow and mature faster but have a shorter lifespan. Based on the differences in expression and solubility of proteins, we established an aging pseudo-time scale. Notably, the newly born ultralight worms appear to be significantly "younger" than their normal counterparts, while at day 10 they are significantly "older". Pathway analysis revealed involvement of mitochondria; analysis of reactive oxygen species (ROS) confirmed significant ROS overproduction in ultralight worms that increases further with age. These findings provide a new modality of affecting the lifespan in this important animal model of human diseases and aging.

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Zhang, X., Gharibi, H., Beusch, C. M., Meng, Z., Saei Dibavar, A., Gaetani, M., Zubarev, R.. 2024-02-13. Do light eaters live shorter lives? The case of ultralight Caenorhabditis elegans. https://doi.org/10.1101/2024.02.13.580069

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