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bioRxiv · 10.1101/2024.02.12.579969

Evaluation of a novel Tc-24 recombinant antigen ELISA for serologic testing for Trypanosoma cruzi in dogs

Mejia, R.·Verocai, G. G.·Mosley, I. A.·Zhan, B.·Vongthavaravat, L.·Busselman, R. E.·Hamer, S. A.
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Abstract

Chagas disease is a parasitic infection caused by Trypanosoma cruzi. Diagnosis of chroni Chagas disease in dogs relies on limited serological test options. This study used a new Tc-24 recombinant antigen ELISA on an archival set of 70 dog serum samples from multi-dog kennel environments in Texas subjected to three existing Chagas serological tests. Tc-24 ELISA produced a quantitative result and could detect anti-T. cruzi antibodies in dogs with high sensitivity and specificity. Comparing individual tests to Tc-24 ELISA resulted in strong associations and correlations, which suggest that Tc-24 ELISA is a reliable and accurate diagnostic tool for dogs with a single test.

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Mejia, R., Verocai, G. G., Mosley, I. A., Zhan, B., Vongthavaravat, L., Busselman, R. E., Hamer, S. A.. 2024-02-14. Evaluation of a novel Tc-24 recombinant antigen ELISA for serologic testing for Trypanosoma cruzi in dogs. https://doi.org/10.1101/2024.02.12.579969

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