Direct, bisulfite-free 5mC and 5hmC sequencing at single-cell resolution

Chen, X.; Cheng, J.; Kong, L.; Shu, X.; Xu, H.; Inoue, M.; Berrocal, M. S. F.; Doskeland, D. S.; Sivakumar, S.; Liu, Y.; Ye, J.; Song, C.-X.

doi:10.1101/2024.02.12.579864

bioRxiv · 10.1101/2024.02.12.579864

Direct, bisulfite-free 5mC and 5hmC sequencing at single-cell resolution

Chen, X.·Cheng, J.·Kong, L.·Shu, X.·Xu, H.·Inoue, M.·Berrocal, M. S. F.·Doskeland, D. S.·Sivakumar, S.·Liu, Y.·Ye, J.·Song, C.-X.

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Abstract

We report the first direct sequencing methodologies for quantitative detection of 5mC and 5hmC at single-base resolution and single-cell level, termed scTAPS (for 5mC + 5hmC) and scCAPS+ (for 5hmC specifically). With [~]90% mapping efficiency, our methods accurately benchmark 5mC and 5hmC profiles in CD8+ T and mES cells, respectively. Notably, scCAPS+ revealed a global increase in 5hmC within the hippocampus of aging mice, both in neurons and in non-neurons.

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Chen, X., Cheng, J., Kong, L., Shu, X., Xu, H., Inoue, M., Berrocal, M. S. F., Doskeland, D. S., Sivakumar, S., Liu, Y., Ye, J., Song, C.-X.. 2024-02-12. Direct, bisulfite-free 5mC and 5hmC sequencing at single-cell resolution. https://doi.org/10.1101/2024.02.12.579864

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