Far-red chemigenetic biosensors for multi-dimensional and super-resolved kinase activity imaging

Frei, M. S.; Sanchez, S. A.; Liu, L.; Schneider, F.; Wang, Z.; Hakozaki, H.; Li, Y.; Lyons, A. C.; Rohm, T. V.; Olefsky, J. M.; Shi, L.; Schöneberg, J.; Fraser, S. E.; Mehta, S.; Wang, Y.; Zhang, J.

doi:10.1101/2024.02.10.579766

bioRxiv · 10.1101/2024.02.10.579766

Far-red chemigenetic biosensors for multi-dimensional and super-resolved kinase activity imaging

Frei, M. S.·Sanchez, S. A.·Liu, L.·Schneider, F.·Wang, Z.·Hakozaki, H.·Li, Y.·Lyons, A. C.·Rohm, T. V.·Olefsky, J. M.·Shi, L.·Schöneberg, J.·Fraser, S. E.·Mehta, S.·Wang, Y.·Zhang, J.

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Abstract

Fluorescent biosensors revolutionized biomedical science by enabling the direct measurement of signaling activities in living cells, yet the current technology is limited in resolution and dimensionality. Here, we introduce highly sensitive chemigenetic kinase activity biosensors that combine the genetically encodable self-labeling protein tag HaloTag7 with bright far-red-emitting synthetic fluorophores. This technology enables five-color biosensor multiplexing, 4D activity imaging, and functional super-resolution imaging via stimulated emission depletion (STED) microscopy.

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Frei, M. S., Sanchez, S. A., Liu, L., Schneider, F., Wang, Z., Hakozaki, H., Li, Y., Lyons, A. C., Rohm, T. V., Olefsky, J. M., Shi, L., Schöneberg, J., Fraser, S. E., Mehta, S., Wang, Y., Zhang, J.. 2024-02-11. Far-red chemigenetic biosensors for multi-dimensional and super-resolved kinase activity imaging. https://doi.org/10.1101/2024.02.10.579766

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