Dual action of sphinganine in the plant disease resistance to bacteria.

Huby, E.; Villaume, S.; Chemotti, C.; Dorey, S.; Cordelier, S.; Crouzet, J.; Gilliard, G.; Terryn, C.; Berquand, A.; Herrfurth, C.; Feussner, I.; Jacquard, C.; Fontaine, F.; Clement, C.; Baillieul, F.; Deleu, M.

doi:10.1101/2024.02.07.579277

bioRxiv · 10.1101/2024.02.07.579277

Dual action of sphinganine in the plant disease resistance to bacteria.

Huby, E.·Villaume, S.·Chemotti, C.·Dorey, S.·Cordelier, S.·Crouzet, J.·Gilliard, G.·Terryn, C.·Berquand, A.·Herrfurth, C.·Feussner, I.·Jacquard, C.·Fontaine, F.·Clement, C.·Baillieul, F.·Deleu, M.

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Abstract

Sphingolipids are ubiquitous, highly diverse molecules constituting at least 40% of plant plasma membranes. Initially known as modulators of membrane integrity, they now emerge as important players in plant responses to (a)biotic stresses. The interaction between Arabidopsis thaliana and the bacterium Pseudomonas syringae pv. tomato DC3000 AvrRpm1 (Pst AvrRpm1) culminates in the activation of a programmed cell death known as the hypersensitive response, which is part of the plant immune response. In this study, we showed that the co-infiltration of Pst AvrRpm1 and sphinganine (d18:0) in Arabidopsis leaves suppress the hypersensitive response. This suppression phenotype is also observed with bacteria carrying the effectors AvrB and AvrPphB but not with the ones carrying AvrRpt2 and AvrRps4. Sphingolipid-induced hypersensitive response suppression by Pst AvrRpm1 is correlated with the down-regulation of the gene AtNMT1 encoding a N-myristoyltransferase. d18:0 does not have a direct antibacterial effect and its co-infiltration in plants does not display typical signs of immune response such as activation of salicylic acid signaling pathway and extracellular reactive oxygen species production. Biophysical studies showed that d18:0 interacts with plant plasma membrane lipids. More specifically, d18:0 disturbs plant plasma membrane organization and mechanical properties. Our results demonstrate that sphingolipids play an important role in plant resistance, especially by interfering with the plasma membrane organization and effector localization and thus disturbing their function and subsequent immune responses.

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Huby, E., Villaume, S., Chemotti, C., Dorey, S., Cordelier, S., Crouzet, J., Gilliard, G., Terryn, C., Berquand, A., Herrfurth, C., Feussner, I., Jacquard, C., Fontaine, F., Clement, C., Baillieul, F., Deleu, M.. 2024-02-08. Dual action of sphinganine in the plant disease resistance to bacteria.. https://doi.org/10.1101/2024.02.07.579277

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