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bioRxiv · 10.1101/2024.02.07.578830

Cytoplasmic protein-free mRNA induces stress granules by two G3BP1/2-dependent mechanisms

Ihn, S. J.·Farlam-Williams, L.·Palazzo, A. F.·Lee, H. O.
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Abstract

Stress granules are cytoplasmic membraneless organelles that sequester proteins and non-translating mRNAs in response to various stressors. To assess the contributions of mRNA and RNA-binding proteins to stress granule formation, we use microinjection to deliver protein-free mRNA into the cytoplasm in a controlled manner. We demonstrate that mRNAs trigger stress granule formation through two mechanisms that are enhanced by the presence of G3BP1 and G3BP2. Low concentrations of in vitro transcribed mRNA activated protein kinase R (PKR), leading to phosphorylation and inhibition of the eukaryotic translation initiation factor eIF2 and stress granule formation. This was inhibited by replacing uridine with pseudouridine in the mRNA or by treating it with RNase III, which cleaves double-stranded RNA. High concentrations of mRNA triggered stress granule formation by a mechanism that was independent of PKR and enhanced by G3BP1/2, highlighting the importance of both protein-free mRNA and RNA-binding proteins in stress granule formation. Graphical Abstract/Model O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=197 SRC="FIGDIR/small/578830v1_ufig1.gif" ALT="Figure 1"> View larger version (27K): org.highwire.dtl.DTLVardef@1b1bcfeorg.highwire.dtl.DTLVardef@1eee33corg.highwire.dtl.DTLVardef@888c9forg.highwire.dtl.DTLVardef@c835aa_HPS_FORMAT_FIGEXP M_FIG C_FIG SummaryMicroinjected mRNA induces stress granules in mammalian cells by two G3BP1/2-dependent mechanisms: one requires the stress-sensing protein kinase PKR to phosphorylate the translation initiation factor eIF2, and the other is independent of PKR and phospho-eIF2 and acts when the cytoplasmic concentration of ribosome-free mRNA is increased acutely.

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Ihn, S. J., Farlam-Williams, L., Palazzo, A. F., Lee, H. O.. 2024-02-08. Cytoplasmic protein-free mRNA induces stress granules by two G3BP1/2-dependent mechanisms. https://doi.org/10.1101/2024.02.07.578830

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