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bioRxiv · 10.1101/2024.02.05.578902

Deciphering SH-SY5Y neuroblastoma cell proliferation: A detailed investigation of seeding concentrations, cultivation surfaces, and long-term maintenance in a sealed chamber

Kalwarczyk, E.·Lukasiak, A.·Woznica, D.·Switlik, W.·Anchimowicz, J.·Zielonka, P.·Jakiela, S.
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Abstract

BackgroundMicrofluidics offers precise drug delivery and continuous monitoring of cell functions, which is crucial for studying the effects of toxins and drugs. Ensuring proper cell growth in these space-constrained systems is essential for obtaining consistent results comparable to standard Petri dishes. New methodWe investigated the proliferation of SH-SY5Y cells on circular polycarbonate chambers with varying surface areas. SH-SY5Y cells were chosen for their relevance in neurodegenerative disease research. ResultsOur study demonstrates a correlation between the chamber surface area and SH-SY5Y cell growth rates. Cells cultured in chambers larger than 10 mm in diameter exhibited growth comparable to standard 60-mm dishes. In contrast, smaller chambers significantly impeded growth, even at identical seeding densities. Similar patterns were observed for HeLaGFP cells, while 16HBE14{sigma} cells proliferated efficiently regardless of chamber size. Additionally, SH-SY5Y cells were studied in a 12-mm diameter sealed chamber to assess growth under restricted gas exchange conditions. Comparison with existing methodsOur findings underscore the limitations of small chamber sizes in microfluidic systems for SH-SY5Y cells, an issue not typically addressed by conventional methods. ConclusionsSH-SY5Y cell growth is highly sensitive to spatial constraints, with markedly reduced proliferation in chambers smaller than 10 mm. This highlights the need to carefully consider chamber size in microfluidic experiments to achieve cell growth rates comparable to standard culture dishes. The study also shows that while SH-SY5Y and HeLaGFP cells are affected by chamber size, 16HBE14{sigma} cells are not. These insights are vital for designing effective microfluidic systems for bioengineering research.

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Kalwarczyk, E., Lukasiak, A., Woznica, D., Switlik, W., Anchimowicz, J., Zielonka, P., Jakiela, S.. 2024-02-08. Deciphering SH-SY5Y neuroblastoma cell proliferation: A detailed investigation of seeding concentrations, cultivation surfaces, and long-term maintenance in a sealed chamber. https://doi.org/10.1101/2024.02.05.578902

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