Age-related RPE Changes in Wildtype C57BL/6J Mice Between 2 and 32 Months

Shelton, D. A.; Gefke, I.; Liao, K.; Summers, V.; Yu, H.; Kim, Y.-K.; Getz, Y.; Ferdous, S.; Donaldson, K.; Papania, J.; Chrenek, M.; Boatright, J.; Nickerson, J.

doi:10.1101/2024.01.30.574142

bioRxiv · 10.1101/2024.01.30.574142

Age-related RPE Changes in Wildtype C57BL/6J Mice Between 2 and 32 Months

Shelton, D. A.·Gefke, I.·Liao, K.·Summers, V.·Yu, H.·Kim, Y.-K.·Getz, Y.·Ferdous, S.·Donaldson, K.·Papania, J.·Chrenek, M.·Boatright, J.·Nickerson, J.

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Abstract

PurposeThis study provides a systematic evaluation of age-related changes in RPE cell structure and function using a morphometric approach. We aim to better capture nuanced predictive changes in cell heterogeneity that reflect loss of RPE integrity during normal aging. Using C57BL6/J mice ranging from P60-P730, we sought to evaluate how regional changes in RPE shape reflect incremental losses in RPE cell function with advancing age. We hypothesize that tracking global morphological changes in RPE is predictive of functional defects over time. MethodsWe tested three groups of C57BL/6J mice (young: P60-180; Middle-aged: P365-729; aged: 730+) for function and structural defects using electroretinograms, immunofluorescence, and phagocytosis assays. ResultsThe largest changes in RPE morphology were evident between the young and aged groups, while the middle-aged group exhibited smaller but notable region-specific differences. We observed a 1.9-fold increase in cytoplasmic alpha-catenin expression specifically in the central-medial region of the eye between the young and aged group. There was an 8-fold increase in subretinal, IBA-1-positive immune cell recruitment and a significant decrease in visual function in aged mice compared to young mice. Functional defects in the RPE corroborated by changes in RPE phagocytotic capacity. ConclusionsThe marked increase of cytoplasmic alpha-catenin expression and subretinal immune cell deposition, and decreased visual output coincide with regional changes in RPE cell morphometrics when stratified by age. These cumulative changes in the RPE morphology showed predictive regional patterns of stress associated with loss of RPE integrity.

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Shelton, D. A., Gefke, I., Liao, K., Summers, V., Yu, H., Kim, Y.-K., Getz, Y., Ferdous, S., Donaldson, K., Papania, J., Chrenek, M., Boatright, J., Nickerson, J.. 2024-02-01. Age-related RPE Changes in Wildtype C57BL/6J Mice Between 2 and 32 Months. https://doi.org/10.1101/2024.01.30.574142

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