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bioRxiv · 10.1101/2024.01.29.577736

Decreasing ganglioside synthesis delays motor and cognitive symptom onset in Spg11 knockout mice

Abstract

Biallelic variants in the SPG11 gene account for the most common form of autosomal recessive hereditary spastic paraplegia characterized by motor and cognitive impairment, with currently no therapeutic option. We previously observed in a Spg11 knockout mouse that neurodegeneration is associated with accumulation of gangliosides in lysosomes. To test whether a substrate reduction therapy could be a therapeutic option, we downregulated the key enzyme involved in ganglioside biosynthesis using an AAV-PHP.eB viral vector expressing a miRNA targeting St3gal5. Downregulation of St3gal5 in Spg11 knockout mice prevented the accumulation of gangliosides, delayed the onset of motor and cognitive symptoms, and prevented the upregulation of serum levels of neurofilament light chain, a biomarker widely used in neurodegenerative diseases. Importantly, similar results were observed upon treatment of Spg11 knockout mice with venglustat, a pharmacological inhibitor of glucosylceramide synthase expected to decrease ganglioside synthesis. Downregulation of St3gal5 or venglustat treatment of Spg11 knockout mice strongly decreased the formation of axonal spheroids, previously associated with impaired trafficking. Venglustat had similar effect on cultured human SPG11 neurons. In conclusion, this work identifies the first disease-modifying therapeutic strategy in SPG11, and provides data supporting its relevance for therapeutic testing in SPG11 patients.

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BibTeXRIS

Fortier, M., Cauhape, M., Buono, S., Becker, J., Menuet, A., Branchu, J., Ricca, I., Mero, S., Dorgham, K., El Hachimi, K. H., Dobrenis, K., Colsch, B., Samaroo, D., Devaux, M., Durr, A., Stevanin, G., Santorelli, F. M., Colombo, S., Cowling, B., Darios, F.. 2024-01-29. Decreasing ganglioside synthesis delays motor and cognitive symptom onset in Spg11 knockout mice. https://doi.org/10.1101/2024.01.29.577736

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