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bioRxiv · 10.1101/2024.01.22.576602

Decoding Cortical Circuits: Synaptic Signatures and Disease Vulnerabilities of Layer 5 Pyramidal Neuron Types

Marcassa, G.·Dascenco, D.·Lorente-Echeverria, B.·Vanderlinden, J.·Leysen, E.·Baltussen, L.·Howden, A. J. M.·de Wit, J.
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Abstract

Cortical layer 5 (L5) intratelencephalic (IT) and pyramidal tract (PT) neurons are embedded in distinct information processing pathways. The morphology, connectivity, electrophysiological properties, and role in behavior of these neurons have been extensively analyzed. However, the molecular composition of their synapses remains largely uncharacterized. Here, we dissect the protein composition of the excitatory postsynaptic compartment of L5 neurons in intact somatosensory circuits, using an optimized proximity biotinylation workflow with subsynaptic resolution. We find distinct synaptic signatures of L5 IT and PT neurons that are defined by proteins regulating synaptic organization and transmission, including cell-surface proteins (CSPs), neurotransmitter receptors and ion channels. In addition, we find a differential vulnerability to disease, with a marked enrichment of autism risk genes in the synaptic signature of L5 IT neurons compared to PT neurons. Our results align with human studies and suggest that the excitatory postsynaptic compartment of L5 IT neurons is notably susceptible in autism. Together, our analysis sheds light on the proteins that regulate synaptic organization and function of L5 neuron types and contribute to their susceptibility in disease. Our approach is versatile and can be broadly applied to other neuron types to create a protein-based, synaptic atlas of cortical circuits.

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Marcassa, G., Dascenco, D., Lorente-Echeverria, B., Vanderlinden, J., Leysen, E., Baltussen, L., Howden, A. J. M., de Wit, J.. 2024-01-22. Decoding Cortical Circuits: Synaptic Signatures and Disease Vulnerabilities of Layer 5 Pyramidal Neuron Types. https://doi.org/10.1101/2024.01.22.576602

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