Chromosomal-level genome assembly of the long-spined sea urchin Diadema setosum (Leske, 1778)

Hong Kong Biodiversity Genomics Consortium,; Hui, J. H. L.; Chan, T.-F.; Chan, L.; Cheung, S. G.; Cheang, C. C.; Fang, J.; Gaitan-Espitia, J. D.; Lau, S.; Sung, Y. H.; Wong, C.; Yip, K.; Wei, Y.; So, W. L.; Nong, W.; Chui, A. P. Y.; Fong, T. H. W.; Yip, H. Y.

doi:10.1101/2024.01.16.575490

bioRxiv · 10.1101/2024.01.16.575490

Chromosomal-level genome assembly of the long-spined sea urchin Diadema setosum (Leske, 1778)

Hong Kong Biodiversity Genomics Consortium,·Hui, J. H. L.·Chan, T.-F.·Chan, L.·Cheung, S. G.·Cheang, C. C.·Fang, J.·Gaitan-Espitia, J. D.·Lau, S.·Sung, Y. H.·Wong, C.·Yip, K.·Wei, Y.·So, W. L.·Nong, W.·Chui, A. P. Y.·Fong, T. H. W.·Yip, H. Y.

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Abstract

The long-spined sea urchin Diadema setosum is an algal and coral feeder widely distributed in the Indo-Pacific and can cause severe bioerosion on the reef community. Nevertheless, the lack of genomic information has hindered the study its ecology and evolution. Here, we report the chromosomal-level genome (885.8 Mb) of the long-spined sea urchin D. setosum using a combination of PacBio long-read sequencing and Omni-C scaffolding technology. The assembled genome contained scaffold N50 length of 38.3 Mb, 98.1 % of BUSCO (Geno, metazoa_odb10) genes, and with 98.6% of the sequences anchored to 22 pseudo-molecules/chromosomes. A total of 27,478 genes including 23,030 protein-coding genes were annotated. The high-quality genome of D. setosum presented here provides a significant resource for further understanding on the ecological and evolutionary studies of this coral reef associated sea urchin.

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Hong Kong Biodiversity Genomics Consortium,, Hui, J. H. L., Chan, T.-F., Chan, L., Cheung, S. G., Cheang, C. C., Fang, J., Gaitan-Espitia, J. D., Lau, S., Sung, Y. H., Wong, C., Yip, K., Wei, Y., So, W. L., Nong, W., Chui, A. P. Y., Fong, T. H. W., Yip, H. Y.. 2024-01-18. Chromosomal-level genome assembly of the long-spined sea urchin Diadema setosum (Leske, 1778). https://doi.org/10.1101/2024.01.16.575490

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