Single Cell Calcium profiling for the discrimination of cancer cell lines.

Caussette, C.; Bura, A.; Gueguinou, M.; Bellanger, D.; Robert, A.; Cancel, M.; Fromont-Hankard, G.; Vandier, C.; Maheo, K.; Brouard, T.; Crottes, D.

doi:10.1101/2024.01.14.575608

bioRxiv · 10.1101/2024.01.14.575608

Single Cell Calcium profiling for the discrimination of cancer cell lines.

Caussette, C.·Bura, A.·Gueguinou, M.·Bellanger, D.·Robert, A.·Cancel, M.·Fromont-Hankard, G.·Vandier, C.·Maheo, K.·Brouard, T.·Crottes, D.

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Abstract

Calcium (Ca2+) is an essential second messenger that controls numerous cellular functions. Characteristics of intracellular Ca2+ oscillations define Ca2+ signatures representatives of the phenotype of a cell. Oncogenic functions such as migration, proliferation or resistance to chemotherapy have been associated with aberrant Ca2+ fluxes. However, the identification of Ca2+ signatures representatives of the oncogenic properties of cancer cells remains to be addressed. To characterize oncogenic Ca2+ signatures, we proposed an unbiased scalable method that combines single cell calcium imaging with unsupervised and supervised machine learning tools. Here, we applied this method to investigate the heterogeneity of Ca 2+ signatures in 16 cancer cell lines and the remodeling of oncogenic Ca2+ signatures induced by acquired docetaxel resistance and in the course of the interaction of cancer cells with fibroblasts. Finally, our method demonstrates the potential of Ca2+ profiling for discriminating cancer cells and predict their phenotypic characteristics at single cell level, and provides a framework for researchers to investigate the remodeling of the Ca2+ signature during cancer development.

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Caussette, C., Bura, A., Gueguinou, M., Bellanger, D., Robert, A., Cancel, M., Fromont-Hankard, G., Vandier, C., Maheo, K., Brouard, T., Crottes, D.. 2024-01-15. Single Cell Calcium profiling for the discrimination of cancer cell lines.. https://doi.org/10.1101/2024.01.14.575608

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