Protein kinase Sut1 from the Crenarchaeon Sulfolobus acidocaldarius displays tyrosine phosphorylation activity

Maklad, H. R.; Ruys, S. P. d.; Bervoets, I.; Vertommen, D.; Gutierrez, G. J.; Vranken, W. F.; Siebers, B.; Peeters, E.

doi:10.1101/2024.01.14.552423

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bioRxiv · 10.1101/2024.01.14.552423

Protein kinase Sut1 from the Crenarchaeon Sulfolobus acidocaldarius displays tyrosine phosphorylation activity

Maklad, H. R.·Ruys, S. P. d.·Bervoets, I.·Vertommen, D.·Gutierrez, G. J.·Vranken, W. F.·Siebers, B.·Peeters, E.

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Abstract

Protein phosphorylation is a key cellular signaling mechanism that exists in all life forms. Unlike Bacteria and Eukarya, in which protein phosphorylation has thoroughly been studied, post-translational modification by means of phosphorylation have only been limitedly explored in Archaea. A previous study of the phosphoproteome of the model Crenarchaeon Sulfolobus acidocaldarius revealed a widespread occurrence of protein phosphorylation, especially on tyrosine residues. Moreover, several (putative) transcription factors, including AbfR1 and FadR, were previously shown to be phosphorylated on tyrosine residues, a phenomenon that is directly linked to a phosphorylation-mediated regulation of these transcription factors. Despite this potentially important role for tyrosine phosphorylation in S. acidocaldarius, to our knowledge, a kinase capable of directly phosphorylating tyrosine residues has not yet been identified in this organism, neither in Archaea as a whole. Here, we identify and characterize a protein kinase in S. acidocaldarius, Sut1, which displays tyrosine phosphorylation activity in vitro and represents a novel protein kinase family that is widespread in different archaeal organisms.

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Maklad, H. R., Ruys, S. P. d., Bervoets, I., Vertommen, D., Gutierrez, G. J., Vranken, W. F., Siebers, B., Peeters, E.. 2024-01-14. Protein kinase Sut1 from the Crenarchaeon Sulfolobus acidocaldarius displays tyrosine phosphorylation activity. https://doi.org/10.1101/2024.01.14.552423

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