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bioRxiv · 10.1101/2024.01.09.574802

De novo annotation of the wheat pangenome reveals complexity and diversity of the hexaploid wheat pan-transcriptome

Abstract

Wheat is the most widely cultivated crop in the world with over 215 million hectares grown annually. However, to meet the demands of a growing global population, breeders face the challenge of increasing wheat production by approximately 60% within the next 40 years. The 10+ Wheat Genomes Project recently sequenced and assembled to chromosome level the genomes of nine wheat cultivars to develop our understanding of genetic diversity and selection within the pan-genome of wheat. Here, we provide a wheat pan-transcriptome with de novo annotation and differential expression analysis for these wheat cultivars, across multiple different tissues and whole seedlings sampled at dusk/dawn. Analysis of these de novo annotations facilitated the discovery of genes absent from the Chinese Spring reference, identified genes specific to particular cultivars and defined the core and dispensable genomes. Expression analysis across cultivars and tissues revealed conservation in expression between a large core set of homeologous genes, but also widespread changes in subgenome homeolog expression bias between cultivars. Co-expression network analysis revealed the impact of divergence of sub-genome homeolog expression and identified cultivar-specific expression profiles. In a case study utilising both the newly constructed wheat pan-genome and pan-transcriptome we demonstrate prevalent variation in the prolamin superfamily and immune-reactive proteins across the pan-cultivars.In summary, this work provides both a valuable resource for the wider wheat community and reveals diversity in gene content and expression patterns between global wheat cultivars.

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White, B., LUX, T., Rusholme-Pilcher, R., Juhasz, A., Kaithakottil, G., Duncan, S., Simmonds, J., Rees, H., Wright, J., Colmer, J., Ward, S., joynson, R., Coombes, B., Irish, N., Henderson, S., Baker, T., Chapman, H., Catchpole, L., Gharbi, K., Okada, M., Handa, H., Nasuda, S., Shimizu, K., Gundlach, H., Lang, D., Naamati, G., Legg, E., Bharti, A., Colgrave, M., Haerty, W. `, Uauy, C. `, Swarbreck, D., Borrill, P., Poland, J., Krattinger, S., Stein, N., Mayer, K. F. X., Pozniak, C., Spannagl, M., Hall, A.. 2024-01-09. De novo annotation of the wheat pangenome reveals complexity and diversity of the hexaploid wheat pan-transcriptome. https://doi.org/10.1101/2024.01.09.574802

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