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bioRxiv · 10.1101/2024.01.03.574123

Fascin structural plasticity mediates flexible actin bundle construction

Gong, R.·Reynolds, M. J.·Hamilton, K.·Alushin, G. M.
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Abstract

Fascin crosslinks actin filaments (F-actin) into bundles that support tubular membrane protrusions including filopodia and stereocilia. Fascin dysregulation drives aberrant cell migration during metastasis, and fascin inhibitors are under development as cancer therapeutics. Here, we use cryo-electron microscopy, cryo-electron tomography coupled with custom denoising, and computational modeling to probe fascins F-actin crosslinking mechanisms across spatial scales. Our fascin crossbridge structure reveals an asymmetric F-actin binding conformation that is allosterically blocked by the inhibitor G2. Reconstructions of seven-filament hexagonal bundle elements, variability analysis, and simulations show how structural plasticity enables fascin to bridge varied inter-filament orientations, accommodating mismatches between F-actins helical symmetry and bundle hexagonal packing. Tomography of many-filament bundles and modeling uncovers geometric rules underlying emergent fascin binding patterns, as well as the accumulation of unfavorable crosslinks that limit bundle size. Collectively, this work shows how fascin harnesses fine-tuned nanoscale structural dynamics to build and regulate micron-scale F-actin bundles.

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Gong, R., Reynolds, M. J., Hamilton, K., Alushin, G. M.. 2024-01-04. Fascin structural plasticity mediates flexible actin bundle construction. https://doi.org/10.1101/2024.01.03.574123

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