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bioRxiv · 10.1101/2023.12.24.573263

Genetic Mapping of Monocyte Fate Decisions Following Myocardial Infarction

Koenig, A. L.·Kadyrov, F. F.·Amrute, J. M.·Yang, S.·Weinheimer, C. J.·Nigro, J. M.·Kovacs, A.·Smith, G.·Lavine, K. J.
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Abstract

Inflammation contributes to the pathogenesis of myocardial infarction and heart failure and represents a viable therapeutic target. Monocytes and their progeny are highly abundant and display incredible functional diversity, serving as key determinants of myocardial inflammation and tissue repair. Much remains to be learned regarding mechanisms and signaling events that instruct monocyte fate decisions. We devised a genetic lineage tracing strategy using Ccr2crERT2Rosa26LSL-tdTomato mice in combination with single cell RNA-sequencing to map the differentiation trajectories of monocytes that infiltrate the heart after reperfused myocardial infarction. Monocytes are recruited to the heart early after injury and give rise to transcriptionally distinct and spatially restricted macrophage and dendritic cell-like subsets that are specified prior to extravasation and chronically persist within the myocardium. Pseudotime analysis predicted two differentiation trajectories of monocyte-derived macrophages that are partitioned into the border and infarct zones, respectively. Among these trajectories, we show that macrophages expressing a type I IFN responsive signature are an intermediate population that gives rise to MHC-IIhi macrophages, are localized within the border zone, and promote myocardial protection. Collectively, these data uncover new complexities of monocyte differentiation in the infarcted heart and suggest that modulating monocyte fate decisions may have clinical implications.

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Koenig, A. L., Kadyrov, F. F., Amrute, J. M., Yang, S., Weinheimer, C. J., Nigro, J. M., Kovacs, A., Smith, G., Lavine, K. J.. 2023-12-25. Genetic Mapping of Monocyte Fate Decisions Following Myocardial Infarction. https://doi.org/10.1101/2023.12.24.573263

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