Automated workflow for the cell cycle analysis of non-adherent and adherent cells using a machine learning approach

Hayatigolkhatmi, K.; Soriani, C.; Soda, E.; Ceccacci, E.; El Menna, O.; Peri, S.; Negrelli, I.; Bertolini, G.; Franchi, G. M.; Carbone, R.; Minucci, S.; Rodighiero, S.

doi:10.1101/2023.12.21.572803

bioRxiv · 10.1101/2023.12.21.572803

Automated workflow for the cell cycle analysis of non-adherent and adherent cells using a machine learning approach

Hayatigolkhatmi, K.·Soriani, C.·Soda, E.·Ceccacci, E.·El Menna, O.·Peri, S.·Negrelli, I.·Bertolini, G.·Franchi, G. M.·Carbone, R.·Minucci, S.·Rodighiero, S.

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Abstract

Understanding the details of the cell cycle at the level of individual cells is critical for both cellular biology and cancer research. While existing methods using specific fluorescent markers have advanced our ability to study the cell cycle in cells that adhere to surfaces, there is a clear gap when it comes to non-adherent cells. In this study, we combine a specialized surface to improve cell attachment, the genetically-encoded FUCCI(CA)2 sensor, an automated image processing and analysis pipeline, and a custom machine-learning algorithm. This combined approach allowed us to precisely measure the duration of different cell cycle phases in non-adherent cells. Our method provided detailed information from hundreds of cells under different experimental conditions in a fully automated manner. We validated this approach in two different Acute Myeloid Leukemia (AML) cell lines, NB4 and Kasumi-1, which have unique cell cycle characteristics. Additionally, we tested the impact of drugs affecting the cell cycle in NB4 cells. Importantly, our cell cycle analysis system is freely available and has also been validated for use with adherent cells. In summary, this report introduces a comprehensive, automated method for studying the cell cycle in both adherent and non-adherent cells, offering a valuable tool for cancer research and drug development.

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Hayatigolkhatmi, K., Soriani, C., Soda, E., Ceccacci, E., El Menna, O., Peri, S., Negrelli, I., Bertolini, G., Franchi, G. M., Carbone, R., Minucci, S., Rodighiero, S.. 2023-12-21. Automated workflow for the cell cycle analysis of non-adherent and adherent cells using a machine learning approach. https://doi.org/10.1101/2023.12.21.572803

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