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bioRxiv · 10.1101/2023.12.15.571855

Production of single-cell-protein (SCP)/poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) matrices through fermentation of archaea Haloferax mediterranei

Unis, R.·Gnaim, R.·Kashyap, M.·Shamis, O.·Gnayem, N.·Gozin, M.·Liberzon, A.·Gnaim, J.·Golberg, A.
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Abstract

The idea of in-situ integrating poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) sieves in a single-cell protein (SCP) represents a promising approach to enhance the properties of microbial biomass as protein alternatives. Archaea SCP/PHBV matrix was successfully produced with a concentration of 8.0 {+/-} 0.1 g L-1 and a productivity of 11.1 mg L-1 h-1 using Haloferax mediterranei. This was achieved by employing 30 g L-1 of enzymatically hydrolyzed bread waste (BW) and 200 g L-1 of red sea salt at 42 {degrees}C and with shaking at 150 rpm for 3 days. The amino acid profile of the SCP/PHBV matrix revealed a total amino acid content of 358 g kg-1, including 147 g kg-1 of essential amino acids. The protein quality of the H. mediterranei SCP/PHBV matrix was assessed using the in-vitro enzyme digestion method, indicating a high-quality protein with an in-vitro digestibility value of 0.91 and a protein digestibility-corrected amino acid score (PDCAAS) of 0.78. The PHBV component (36.0 {+/-} 6.3% w/w) in the SCP/PHBV matrix consisted of a copolymer of 3-hydroxybutyrate and 3- hydroxyvalerate in a 91:9 mol% ratio, respectively. The simultaneous production of PHBV polymeric sieves within the H. mediterranei SCP/PHBV matrix provides an alternative protein source with enhanced physicochemical and thermal properties. HighlightsO_LISCP/PHBV matrices were produced from wasted bread by archaea H. mediterranei. C_LIO_LIThis is the first report that explored the production and properties of SCP/PHBV. C_LIO_LIThe presence of PHBV in SCP affected its physicochemical and thermal properties. C_LIO_LISCP/PHBV with high-quality protein was achieved with a PDCAAS value of 0.78. C_LI Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=91 SRC="FIGDIR/small/571855v1_ufig1.gif" ALT="Figure 1"> View larger version (22K): org.highwire.dtl.DTLVardef@773447org.highwire.dtl.DTLVardef@94a96org.highwire.dtl.DTLVardef@1ffdfb8org.highwire.dtl.DTLVardef@1b8e27a_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Unis, R., Gnaim, R., Kashyap, M., Shamis, O., Gnayem, N., Gozin, M., Liberzon, A., Gnaim, J., Golberg, A.. 2023-12-15. Production of single-cell-protein (SCP)/poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) matrices through fermentation of archaea Haloferax mediterranei. https://doi.org/10.1101/2023.12.15.571855

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