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bioRxiv · 10.1101/2023.12.14.571754

Targeting HLA-E Positive Cancers with a Novel NKG2A/C Switch Receptor

Abstract

HLA-E is overexpressed by approximately 80% of solid tumors, including malignant glioblastoma, and is emerging as a major checkpoint for NKG2A+ CD8+ T cells and NK cells in the tumor microenvironment and circulation. This axis operates side-by-side with PD-L1 to shut down effector responses by T and NK cells. Here, we engineered a novel chimeric A/C switch receptor, combining the strong HLA-E binding affinity of the NKG2A receptor ectodomain with the activating signaling of the NKG2C receptor endodomain. We found that A/C Switch-transduced NK and T cells displayed superior and specific cytotoxic function when challenged with tumor cells exhibiting medium to high HLA-E expression. Furthermore, A/C Switch-expressing human T cells demonstrated enhanced anti-tumor function in a xenograft model of glioblastoma. Importantly, the activity of the modified T cells was governed by an equilibrium between A/C Switch transduction level and HLA-E expression, creating a therapeutic window to safeguard against on-target off-tumor toxicities. Indeed, normal cells remained insensitive to A/C Switch engineered T cells even after pre-treatment with IFN-{gamma} to induce HLA-E expression. We propose that this novel A/C switch receptor may operate alone to control tumor cells expressing high levels of HLA-E or in combination with other engineered specificities to overcome the suppressive NKG2A/HLA-E checkpoint.

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BibTeXRIS

Saetersmoen, M. L., Kotchetkov, I., Torralba-Raga, L., Masilla-Soto, J., Sohlberg, E., Krokeide, S. Z., Hammer, Q., Sadelain, M., Malmberg, K.-J.. 2023-12-15. Targeting HLA-E Positive Cancers with a Novel NKG2A/C Switch Receptor. https://doi.org/10.1101/2023.12.14.571754

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