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bioRxiv · 10.1101/2023.12.06.569891

SOLUBILIZER OF BACTERIAL ORIGIN SURFACTIN INCREASES THE BIOLOGICAL ACTIVITY OF C60 FULLERENE

Emelyantsev, S.·Prazdnova, E.·Chistyakov, V.
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Abstract

Currently, there exists conflicting data regarding the biological activity of unmodified fullerene C60. Various sources report its toxicity, geroprotective activity, and potential interaction with DNA. Contradictory findings regarding the toxicity of C60 may arise from the use of toxic solvents, as well as the influence of bioavailability and bioactivity on the preparation conditions of C60 suspensions. Furthermore, the microbiota of experimental animals can impact geroprotective activity results by releasing surfactants that facilitate substance penetration through the cell membrane. In this study, we selected conditions for solubilizing fullerene C60 in a solution of surfactin, a surfactant of bacterial origin, as well as in a 2% aqueous solution of TWEEN 80, employing ultrasound. Through bioluminescent analysis using lux biosensors in E. coli MG1655, we observed that C60 in surfactin reduced induced genotoxic and oxidative stress. Given that surfactin enhances membrane permeability to fullerene C60, suspensions of fullerene in designated concentrations of surfactin can be regarded as a DNA protector and antioxidant, warranting further investigation as a promising component of novel drugs.

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Emelyantsev, S., Prazdnova, E., Chistyakov, V.. 2023-12-08. SOLUBILIZER OF BACTERIAL ORIGIN SURFACTIN INCREASES THE BIOLOGICAL ACTIVITY OF C60 FULLERENE. https://doi.org/10.1101/2023.12.06.569891

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