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bioRxiv · 10.1101/2023.12.05.570120

GeneExt: a gene model extension tool for enhanced single-cell RNA-seq analysis

Zolotarov, G.·Grau-Bove, X.·Sebe-Pedros, A.
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Abstract

Incomplete gene models negatively impact single-cell gene expression quantification. This is particularly true in non-model species where often gene 3' ends are inaccurately annotated, while most scRNA-seq methods only capture the 3' transcript region. This results in many genes being incorrectly quantified or not detected. GeneExt leverages scRNA-seq data to refine gene annotations, enhancing biological interpretation and cross-species comparisons of cell type expression atlases.

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Zolotarov, G., Grau-Bove, X., Sebe-Pedros, A.. 2023-12-07. GeneExt: a gene model extension tool for enhanced single-cell RNA-seq analysis. https://doi.org/10.1101/2023.12.05.570120

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