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bioRxiv · 10.1101/2023.12.02.569699

Histone marks enable formation of immiscible phase-separated chromatin compartments

Sun, W.·Wang, J.·Liu, W.·Tang, B.·Qu, H.·Li, H.·Xie, W.·Li, P.
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Abstract

Eukaryotic chromatin is organized into compartments for gene expression regulation, but the underlying mechanisms remain unclear. Here, we demonstrate that multivalent H3K27me3 and its reader, CBX7-PRC1 complex, regulate facultative heterochromatin via a phase separation mechanism, similar to constitutive heterochromatin1. Facultative and constitutive heterochromatin represent distinct, coexisting condensates in nuclei. In vitro, H3K27me3- and H3K9me3-marked nucleosomal arrays and their reader complexes can phase separate into immiscible condensates that are analogous to the relationship between facultative and constitutive heterochromatin in vivo. Moreover, overexpression of CBX7-PRC1 causes aberrant chromatin compartmentalization in vivo as demonstrated by H3K9me3 CUT&Tag and up-regulation of genes related to cancer such as acute myeloblastic leukemia (AML). CBX7 inhibitor effectively inhibits cancer cell proliferation possibly through phase separation-mediated compartment reorganization. Our data demonstrated how the specificity of compartmentalization is achieved based on the formation of immiscible phase-separated condensates, and offer novel epigenetic mechanistic insights into tumor development.

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Sun, W., Wang, J., Liu, W., Tang, B., Qu, H., Li, H., Xie, W., Li, P.. 2023-12-02. Histone marks enable formation of immiscible phase-separated chromatin compartments. https://doi.org/10.1101/2023.12.02.569699

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