Demonstration of Bioplastic Production from CO2 and Formate using the Reductive Glycine Pathway in E. coli

Fedorova, D.; Ben-Nissan, R.; Milshtein, E.; Jona, G.; Dezorella, N.; Feiguelman, G.; Fedorov, R.; Gomaa, A.; Lindner, A. B.; Noor, E.; Milo, R.

doi:10.1101/2023.12.02.569694

bioRxiv · 10.1101/2023.12.02.569694

Demonstration of Bioplastic Production from CO2 and Formate using the Reductive Glycine Pathway in E. coli

Fedorova, D.·Ben-Nissan, R.·Milshtein, E.·Jona, G.·Dezorella, N.·Feiguelman, G.·Fedorov, R.·Gomaa, A.·Lindner, A. B.·Noor, E.·Milo, R.

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Abstract

There is a strong need to develop technologies that reduce anthropogenic pollution and the dependence on nonrenewable Earth resources. One way of doing so is by harnessing biological systems for replacing the production of fossil-fuel based goods with low-environmental-impact alternatives. Recently, progress was made in engineering the model organism E. coli to grow using CO2 and formate as its only carbon and energy sources using the reductive glycine pathway (rGlyP). Here, we use this engineered strain of E. coli as a host system for the production of polyhydroxybutyrate (PHB), a biologically derived and biodegradable plastic. We confirmed the production of PHB in this strain using Nile red fluorescent microscopy, transmission electron microscopy, and GC measurements. Since formate can be efficiently generated from CO2 by electrochemical reduction using renewable energy sources, this study serves as a proof of concept for the emerging field of electro-bioproduction.

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Fedorova, D., Ben-Nissan, R., Milshtein, E., Jona, G., Dezorella, N., Feiguelman, G., Fedorov, R., Gomaa, A., Lindner, A. B., Noor, E., Milo, R.. 2023-12-03. Demonstration of Bioplastic Production from CO2 and Formate using the Reductive Glycine Pathway in E. coli. https://doi.org/10.1101/2023.12.02.569694

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